Meloidogyne incognita is a wide-spread and damaging pathogen of many important crops in the southern United States, and most sorghum genotypes allow significant levels of reproduction by the nematode. A series of greenhouse evaluations were conducted to determine whether a quantitative trait locus (QTL) that imparts a high level of resistance to Meloidogyne incognita in sorghum can effectively be transferred into diverse sorghum genotypes using marker assisted selection. Using marker-assisted selection, the resistance QTL, QTL-Sb.RKN.3.1, from 'Honey Drip' sorghum was crossed into five different sorghum backgrounds that included forage, sweet, and grain sorghum until the BC1F6 generation. Repeated greenhouse experiments documented that the recurrent parent genotypes were all susceptible to M. incognita and statistically similar to each other. In contrast, the BC1F6 genotypes were all highly resistant and similar to each other and similar to the resistant standard, 'Honey Drip'. These results suggest that this resistance QTL could be introgressed using marker assisted selection into many sorghum genotypes and confer a high level of resistance to M. incognita. Thus, this QTL and its associated markers will be useful for sorghum breeding programs to incorporate M. incognita resistance into their sorghum lines.