A method has been developed to measure root intersection density (RID) on a trench-profile in field conditions. Here we describe how 2D spatial distribution mapping of RID can be processed and converted into root length density (RLD) and root distances (ARD) using a new freeware named RACINE2.2. The software also allows a simple modeling of potential root extraction ratio in the soil (PRER). The software contains models calculating RLD, ARD, and PRER from RID for several crops (maize, sorghum, sugarcane, rice, pearl millet, pineapple, eucalyptus). Models may be changed or added into RACINE2.2. RLD, ARD, and PRER are calculated for each spatial unit and can be used to generate 2D maps using RACINE2.2. Data can be exported to a spreadsheet or a surface mapping software for further analysis. It is also possible to import data into RACINE2.2 from a spreadsheet. This application thus makes studies about root-soil interactions, root growth, and root uptake easier. It opens new avenues to characterize root systems to improve root water and nutrient uptake in field conditions.