Chromosome-scale assembly of the sorghum Tx430 genome using Nanopore sequencing and DLS optical mapping

A new chromosome-scale assembly of the sorghum genome was generated by combining Nanopore sequencing with DLS optical mapping.

In this paper, published in this week’s Nature Communications issue, Oxford Nanopore sequences generated on a MinION sequencer and combined them with Bionano Genomics Direct Label and Stain (DLS) optical maps to generate a chromosome-scale de novo assembly of the repeat-rich Sorghum bicolor Tx430 genome. The final assembly consists of 29 scaffolds, encompassing in most cases entire chromosome arms. It has a scaffold N50 of 33.28 Mbps and covers 90% of the expected genome length. A sequence accuracy of 99.85% is obtained after aligning the assembly against Illumina Tx430 data and 99.6% of the 34,211 public gene models align to the assembly. Comparisons of Tx430 and BTx623 DLS maps against the public BTx623 v3.0.1 genome assembly suggest substantial discrepancies whose origin remains to be determined.

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